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Form 24F-2NT MUTUAL FUND SERIES TRUST For: Mar 31

June 21, 2021 10:04 AM EDT
U.S. SECURITIES AND EXCHANGE COMMISSION
Washington, D.C.  20549

FORM 24F-2
Annual Notice of Securities Sold
Pursuant to Rule 24f-2

Read instructions at end of form before preparing form.
Please print or type.


1.Name and address of issuer: 		        Mutual Fund Series Trust
						80 Arkay Drive, Suite 110
						Hauppauge, NY 11788
 
2. Name of each series or class of funds for which this notice is  
   filed (If the form is being filed for all series and classes of
   securities of the issuer, check the box but do not list
   series or classes):  AlphaCentric Income Opportunities Fund
			AlphaCentric Premium Opportunity Fund
			AlphaCentric Robotics and Automation Fund
			AlphaCentric Symmetry Strategy Fund
			AlphaCentric LifeSci Healthcare Fund
 			AlphaCentric Municipal Opportunities Fund
			AlphaCentric Energy Income Fund
  							
3.	Investment Company Act File Number:	811-21872

	Securities Act File Number:		333-132541

4(a)	Last day of fiscal year for which this notice is filed:
					      March 31, 2021

45(b)	Check box if this notice is being filed late (i.e., more
      than 90 Calendar days after the end of issuers fiscal
	year, (See Instruction A,2)


        Note: If the Form is being filed late, Interest must be
              paid on the registration fee due.

4(c)	Check box if this is the last time issuer will be filing
 	this form.	

5. Calculation of registration fee:


(i) Aggregate sale price of securities sold during the 
fiscal year pursuant to section 24(f):               $    2,592,476,099

(ii) Aggregate price of securities redeemed or
repurchased during the fiscal year:	             $    1,547,345,211

(iii) Aggregate price of securities redeemed or 
repurchased during any prior fiscal year ending
no earlier than October 11, 1995 that were not
previously used to reduce registration fees 
payable to the Commission:	                     $    0 

(iv) Total available redemption credits 
(add Items 5(ii)and 5(iii):	                     $     1,547,345,211
                                                                     
(v) Net sales - If Item 5(i) is greater
 than Item 5(iv)[subtract Item 5(iv) from Item 5(i)] $     1,045,130,889
(vi) Redemption credits available for use 
in future years.				     

- if Item 5(i) is less than Item 5(iv) [subtract
Item 5(iv) from Item 5(i)]:			     $       0


(vii) Multiplier for determining registration fee (See
Instruction C.9): 			             X .0001091

(viii) Registration fee due [multiply Item 5(v) by Item
5(vii)] (enter "0" of no fee is due):  	       = $               114,023.78



6. Prepaid Shares
 
   If the response to Item 5(I) was determined by deducting an
   amount of securities that were registered under the Securities
   Act of 1933 pursuant to rule 24e-2 as in effect before
   October 11, 1997, then report the amount of securities (number
   of shares or other units) deducted here: _____0______. If
   there is a number of shares or other units that were registered
   pursuant to Rule 24e-2 remaining unsold at the end of the
   fiscal year for which this form is filed that are available
   for use by the issuer in future fiscal years, then state
   the number here:____0______


7. Interest due - If this form is being filed
   more than 90 days after the end of the issuer's 
   Fiscal year (see Instruction D):             +  $             0



8. Total of the amount of the registration fee due
   plus any interest due [line 5(viii) plus line 7]:
                                                = $   		 114,023.78

9. Date the registration fee and any interest payment was sent to
   the Commission's lockbox depository: 

Method of Delivery:

 [X]     Wire Transfer	

 [ ]     Mail or other means


SIGNATURES

	This report has been signed below by the following persons
        on behalf of the issuer and in the capacities and on the
        dates indicated.

	By (Signature and Title)* /s/ Erik Naviloff, Treasurer
					  	
	Date	June 21, 2021
	   
	 *Please print the name and title of the signing officer
        below the signature.



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